Viscotek TDA 305

The standard for GPC/SEC with integrated refractive index viscometer and light scattering detectors.

  • Malvern : Viscotek TDA 305

Research grade temperature controlled GPC/SEC detector module to measure sample concentration, molecular weight and intrinsic viscosity. The TDA is ideal for characterizing synthetic and natural polymers and proteins.

OverviewHow it WorksSpecification

Sold as part of the Viscotek TDAmax system, or as a stand-alone module, the Viscotek Triple Detector Array (TDA) incorporates RI, Light Scattering, Viscosity detectors. The integrated detectors and columns are fully temperature controlled up to 80°C to ensure optimum detector stability, and the detectors being series ensures maximum sensitivity. Each detector provides complimentary data on the macromolecules being analyzed.
In its Tetra Detector format, the addition of an ultraviolet phtodiode array (UV-PDA) detector provides additional compositional data of copolymers and conjugated proteins.
Together, the detectors are capable of measuring concentration, molecular weight, intrinsic viscosity and molecular size Rh and Rg of natural and synthetic polymers and proteins. Applications include studying molecular weight, aggregation, conjugates and copolymers, and molecular structure.

  •     Right angle light scattering (RALS) has highest signal to noise for absolute molecular weight of small polymers and proteins.
  •     Absolute molecular weight of synthetic and natural polymers without extrapolation using Low angle light scattering (LALS).
  •     Protein and polymer structure (including branching) information using intrinsic viscosity detector.
  •     High-sensitivity RI detector specifically designed with multi-detection GPC/SEC in mind.
  •     Molecular size in terms of hydrodynamic radius (Rh) and Rg.
  •     Copolymer or protein conjugate composition using multi-wavelength UV-PDA detector
  •     21 CFR part 11 compliant.
  •     Full suite of calibration and GPC/SEC calculation types.

Mobile phase (and sample) enters the TDA and passes through the GPC/SEC chromatography columns. The columns are maintained at the same temperature as the detectors. After eluting from the column, the dissolved polymer molecules, now separated by size, pass through the UV detector (if present), followed by the light scattering and RI detectors. Finally, the mobile phase passes through the viscometer before going to waste.

The RI and UV detectors give information about the concentration of the components in the sample. The light scattering detectors respond to the intensity of light scattered by the sample which is related to molecular weight and also allows Rg of large molecules to be calculated. The viscometer measures the changing solution viscosity to calculate the intrinsic viscosity of the sample which is a measure of structure. By combining the molecular weight and intrinsic viscosity data, the hydrodynamic diameter of the sample can also be calculated along with information on branching.

General

Detector:    Viscotek TDA 305
Dimensions (W, D, H):    41cm x 54cm x 28cm
Weight:    28.5 kg
Laser safety:    Class 1
Software:    OmniSEC, 6 channels at 5Hz, unlimited acquisition time

Detector 1

Detector:    Light Scattering detector
Temperature range:    Ambient to 80°C, all detector cells and columns
Column capacity:    Up to 5 x 30cm columns
Interface:    6 channel digital to OmniSEC software, 2 analogue inputs for exterior RI and UV/LS
Dimensions (W, D, H):    41cm x 54cm x 28cm
Measurement principle:    RALS, 90° angle; LALS, 7° angle, with high efficiency optics
Light source:    Temperature controlled laser diode, 3mW, 670nm
Cell:    Ultra low volume 18µL
Data acquisition rate:    100Hz collection, DSP with 5Hz to OmniSEC

Detector 2

Detector:    Refractive index detector
Measurement principle:    Deflection
Light source:    LED
Cell:    45° quartz glass, 12µL volume
Purging:    3 modes, Manual, Automatic and Programmable
Data acquisition rate:    100Hz collection, DSP with 5Hz to OmniSEC

Detector 3

Detector:    Viscometer Detector
Measurement principle:    Inert, 4 capillary differential Wheatstone bridge configuration
Cell volume:    18µL / capillary
Transducers:    Digital Inert Transducer technology with over-pressure protection
Measurement principle:    18µL
Purging:    3 modes, Manual, Automatic and Programmable
Data acquisition rate:    100Hz collection, DSP with 5Hz to OmniSEC

Detector 4 (option)

Detector:    UV-PDA
Wavelength:    UV (190 to 500nm) OR Vis (430 – 710nm) on 256 diode array
Light source:    Deuterium (UV), Tungsten halogen (Vis)
Cell volume:    10µL
Data acquisition rate:    256 channels with 1Hz to OmniSEC

Measurement type 1

Measurement type:    Absolute Molecular Weight
Measurement range:    <1kDa – 5×10<sup>6</sup> g/mol
Measurement principle:    Light scattering (LALS or RALS)

Measurement type 2

Measurement type:    Intrinsic Viscosity
Measurement range:    0.01 – 20 dL/g
Measurement principle:    4-capillary wheatstone bridge

Measurement type 3

Measurement type:    Concentration
Measurement principle:    Differential Refractive Index detection

Operating environment

Temperature:    15°C – 40°C
Humidity:    35% – 80% non-condensing