{"id":2147,"date":"2020-07-14T15:35:27","date_gmt":"2020-07-14T08:35:27","guid":{"rendered":"http:\/\/www.sujipuli-tech.com\/?page_id=2147"},"modified":"2020-07-23T12:31:33","modified_gmt":"2020-07-23T05:31:33","slug":"dolomite-bio","status":"publish","type":"page","link":"http:\/\/www.sujipuli-tech.com\/?page_id=2147","title":{"rendered":"Nadia Instrument \ufeff"},"content":{"rendered":"\n<p><strong>The Nadia Instrument is an automated, microfluidic droplet- based platform for single cell research that encapsulates up to 8 samples, in parallel, in under 20 mins. Over 50,000 single cells can be captured per cartridge in a run. The fully automated Nadia Instrument guides users through all relevant steps of the experiment via an easy-to-use touchscreen interface. The Nadia can be used with customer supplied reagents or Dolomite Bio\u2019s scRNA-Seq Reagent Kit<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image is-resized\"><img decoding=\"async\" loading=\"lazy\" src=\"http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/nadia1-860x1024.png\" alt=\"\" class=\"wp-image-2149\" width=\"538\" height=\"640\" srcset=\"http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/nadia1-860x1024.png 860w, http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/nadia1-252x300.png 252w, http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/nadia1-768x914.png 768w, http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/nadia1-126x150.png 126w, http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/nadia1.png 1241w\" sizes=\"(max-width: 538px) 100vw, 538px\" \/><figcaption>Nadia Instrument<\/figcaption><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Benefits <\/strong> <\/h2>\n\n\n\n<p><strong>Automation: <\/strong>Fully automated\nsample encapsulation. <\/p>\n\n\n\n<p><strong>Ease of use: <\/strong>Automatic\ndetection of application- specific cartridges, touch screen interface and\nsample loading guide lights. <\/p>\n\n\n\n<p><strong>Scalability: <\/strong>Processing\ncapability of up to 8 samples in parallel. <\/p>\n\n\n\n<p><strong>Temperature\ncontrol: <\/strong>Automated sample chilling to maintain transcriptome state. <\/p>\n\n\n\n<p><strong>Single use\ncartridge: <\/strong>Disposable cartridges with no wetted parts to avoid cross\ncontamination. <strong>Truly single cell: <\/strong>Ultra low cell doublet rates\ndue to gentle cell agitation. \n\n\n\n<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Features <\/strong><\/h2>\n\n\n\n<ul><li>3 independent ultra- smooth pressure pumps each u<\/li><li>Sample temperature control from 4\u00b0C to 40\u00b0C <\/li><li>Independent gentle stirring of beads and cells prior to encapsulation <\/li><li>Easy-to-use integrated touch screen interface <\/li><li>Step-by-step tutorial software <\/li><li>Disposable cartridges prevent cross contamination <\/li><li>Automatic detection of application-specific cartridges <\/li><li>Compatible with Dolomite Bio\u2019s scRNA-Seq Reagent Kit or customer supplied reagents <\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Why use high throughput single cell profiling? <\/strong><\/h2>\n\n\n\n<figure class=\"wp-block-embed-youtube wp-block-embed is-type-video is-provider-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"The Nadia -  An innovation in single cell profiling\" width=\"900\" height=\"506\" src=\"https:\/\/www.youtube.com\/embed\/gTjWVhvBVGA?start=15&#038;feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<p><strong>Techniques\nsuch as high throughput single cell profiling offer the unique capability<br>\nof obtaining qualitative and quantitative transcriptome information from single\ncells. <\/strong>\n\nWith this technology, single\ncells from heterogenous samples, including cultured cells, biopsies, blood and\nother tissues, can be rapidly profiled for quantification of gene expression and\nidentification of specific cells or cell sub-types.\n\n\n\n<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" loading=\"lazy\" width=\"573\" height=\"1024\" src=\"http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/scRNA-Seq-Workflow-with-Nadia-573x1024.png\" alt=\"\" class=\"wp-image-2168\" srcset=\"http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/scRNA-Seq-Workflow-with-Nadia-573x1024.png 573w, http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/scRNA-Seq-Workflow-with-Nadia-168x300.png 168w, http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/scRNA-Seq-Workflow-with-Nadia-768x1371.png 768w, http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/scRNA-Seq-Workflow-with-Nadia-84x150.png 84w, http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/scRNA-Seq-Workflow-with-Nadia.png 1180w\" sizes=\"(max-width: 573px) 100vw, 573px\" \/><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Nadia technology highlights<\/strong><\/h2>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignleft is-resized\"><img decoding=\"async\" loading=\"lazy\" src=\"http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/n3.png\" alt=\"\" class=\"wp-image-2153\" width=\"382\" height=\"382\" srcset=\"http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/n3.png 500w, http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/n3-150x150.png 150w, http:\/\/www.sujipuli-tech.com\/wp-content\/uploads\/2020\/07\/n3-300x300.png 300w\" sizes=\"(max-width: 382px) 100vw, 382px\" \/><figcaption>Nadia cartridge chip <\/figcaption><\/figure><\/div>\n\n\n\n<p><strong>Pressure\npumps <\/strong><\/p>\n\n\n\n<p>Ultra-smooth\npressure pumps for monodisperse droplet formation and superior single capture. <\/p>\n\n\n\n<p><strong>Cell\nstirrer <\/strong><\/p>\n\n\n\n<p>In-built\nstirrers for gentle cell agitation to minimize cell doublet formation and\nblockages. <\/p>\n\n\n\n<p><strong>Bead\nstirrers <\/strong><\/p>\n\n\n\n<p>Continuous\nstirring to ensure singulation and an even distribution of beads throughout the\nencapsulation process. <\/p>\n\n\n\n<p><strong>Guide\nlights <\/strong><\/p>\n\n\n\n<p>Sample\nloading guidelights and touch screen interface to prevent user errors. <\/p>\n\n\n\n<p><strong>Temperature\ncontroller <\/strong><\/p>\n\n\n\n<p>The\nin-built temperature controller chills samples to maintain the cell\ntranscription profile and minimize cell doublet formation. <\/p>\n\n\n\n<p><strong>High\nthroughput droplet junction <\/strong>\n\nOver 50,000 single cells can\nbe captured per cartridge in a run. Live junction visualization is enabled when\nusing the Nadia Innovate.\n\n\n\n<\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Nadia Instrument is an automated, microfluidic droplet- based platform for single cell research that encapsulates up to 8 samples, in parallel, in under 20 mins. Over 50,000 single cells can be captured per cartridge in a run. The fully automated Nadia Instrument guides users through all relevant steps of \u2026<\/p>\n<p class=\"continue-reading-button\"> <a class=\"continue-reading-link\" href=\"http:\/\/www.sujipuli-tech.com\/?page_id=2147\">Continue reading<i class=\"crycon-right-dir\"><\/i><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":295,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v19.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Nadia Instrument \ufeff - Sujipuli Technology<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"http:\/\/www.sujipuli-tech.com\/?page_id=2147\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Nadia Instrument \ufeff - Sujipuli Technology\" \/>\n<meta property=\"og:description\" content=\"The Nadia Instrument is an automated, microfluidic droplet- based platform for single cell research that encapsulates up to 8 samples, in parallel, in under 20 mins. 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Over 50,000 single cells can be captured per cartridge in a run. 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